|
|
|
Registros recuperados: 29 | |
|
|
Durand, Patrick. |
Different parameters have been followed regularly at various times of anchovy (Engraulis encrasicholus ) ripening. Concentration in NaCl and water content as well as values of water activity and pH are constant and remain quite stable during the entire study period. The relatively acid pH value and the quite low measured water activity level created good conditions for preventing microbial putrefaction at room temperature. The free amino acids and non proteinaceous nitrogen increase indicated the modifications occurring in muscular proteins. These changes resulted in an important proteolytic activity modulated by the medium conditions, especially; pH, temperature and ionic strength. The enzymatic activity slowed down regularly with time but was not... |
Tipo: Text |
Palavras-chave: Enzymatic activity; Nitrogen compounds; PH; Biochemical composition; Cured products. |
Ano: 1981 |
URL: http://archimer.ifremer.fr/doc/1981/publication-1889.pdf |
| |
|
| |
|
| |
|
|
Galgani, Francois; Benyamin, Y; Vanworhmoudt, A; Cecaldi, J. |
Proteolytic enzymes of hepatopancreas of six species of peneids (Penaeus kerathurus, P. japonicus, P. monodon, P. stylirostris, P. merguiensis and P. vannamei ) have been studied. Some of their physiological variations have been characterised. Increase in acclimation temperature of crustaceans enhances increase of specific activities of proteases. Variations of salinity does not modify specific activities, nor quantities of trypsin, nor electrophoretic patterns of these different proteases. |
Tipo: Text |
Palavras-chave: Penaeus kerathurus; Penaeidae; Malacostraca; Diets; Growth; Larval development; Life cycle; Acclimation; Environmental effects; Digestive glands; Enzymatic activity; Salinity effects; Temperature effects; Enzymes; Endocrinology; Digestion; Développement larvaire; Croissance; Cycle d'intermue; Carboxypeptidase A and B; Leucine aminopeptidase; Chymotrypsin; Trypsine; Enzymes digestives; Homarus ganmarus; Palaemon serratus; P. vannamei; P. merguiensis; P. stylirostris; P. monodon; P. japonicus; Penaeus keratkurus. |
Ano: 1983 |
URL: http://archimer.ifremer.fr/doc/1983/acte-1195.pdf |
| |
|
|
Kim, S; Hoppe, H. |
A rapid and sensitive method to detect the extracellular enzymatic activity of bacteria colonies grown on agar plates is described. Selective agar media supplemented with protein, starch, chitin, Tween-80, etc. are conventionally used to detect biochemical properties of bacteria. It has been experimentally demonstrated with bacteria pure cultures that fluorogenic Methylumbelliferyl (MUF)-substrates are excellent substrate analogues for normally occurring polymers. Based on MUF-substrate hydrolysis the new method provides reliable qualitative estimates of extracellular enzymatic properties of bacteria within minutes using pure cultures as well as agar plates prepared for colony counts. |
Tipo: Text |
Palavras-chave: Bacteria; Microbiological culture; Biochemical analysis; Enzymatic activity; Analytical techniques. |
Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-959.pdf |
| |
|
|
Meyerreil, L; Graf, G. |
The seasonal development of benthic communities was followed in a coastal marine sediment of the Kiel Bight (Baltic Sea; FRG). Total benthic biomass, (ATP), bacterial biomass, overall benthic activity (heat production), and enzymatic decomposition rates of carbohydrate (d-amylase activity) followed a seasonal cycle strongly related to certain events in the sediment. Among these, the input of the phytoplankton blooms in autumn and spring, the accumulation of organic material during winter, and changes in the physical characteristics of the sediment turned out to be most important for the development of benthic biomass and activity. Processes within the benthic community occurred in very short time scales (within days). |
Tipo: Text |
Palavras-chave: Bacteria; Organic matter; Sediments; Bioenergetics; Enzymatic activity; Biomass. |
Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-944.pdf |
| |
|
| |
|
| |
|
|
CABELLO-PASINI,ALEJANDRO; ALBERTE,RANDALL S.. |
Carbon is acquired through photosynthetic and non-photosynthetic processes in marine algae. However, little is known about the biochemical regulation of these metabolic pathways along the thallus of seaweeds. Consequently, the objective of this study was to assess the distribution of in vivo carboxylation pathways and to relate them to the in vitro activity of ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO), phosphoenolpyruvate carboxykinase (PEPCK), and phosphoenolpyruvate carboxylase (PEPC) in the Phaeophyte Laminaria setchellii, the Chlorophyte Ulva lactuca, and the Rhodophyte Iridaea cordata. Chlorophyll-a levels did not vary in U. lactuca and I. cordata. However, pigment levels were significantly lower in the meristematic region of L.... |
Tipo: Journal article |
Palavras-chave: Enzymatic activity; Chlorophyll; Light-independent carbon fixation; Photosynthesis. |
Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2001000200002 |
| |
|
| |
|
| |
|
|
Raposo,Roberto Wagner Cavalcanti; Muraoka,Takashi; Basso,Luiz Carlos; Lavres Jr.,José; Franzini,Vinicius Ide. |
The phosphate fertilization represents the most costly fraction of soybean crop production. Efficient soybean cultivars for P absorption and utilization in soils of medium available P are highly desirable and might contribute for increasing crop production potential. Thirty two soybean [Glycine max (L.) Merr.] cultivars recommended for 'Cerrado' and differing in growth cycle (early, semi-early, semi-late, and late) were grown in a dystrophic Typic Haplustox Cerrado soil to evaluate the acid phosphatase activity, P content in the diagnostic leaf, and shoot biomass. There were differences among the soybean cultivars within all maturation groups in acid phosphatase activity and shoot biomass. The diagnostic-leaf P-content showed significant differences on... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzymatic activity; Diagnostic-leaf; Maturation group. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000400014 |
| |
|
|
Bravo Rodríguez,Vicente; Jurado Alameda,Encarnación; Martínez Gallegos,Juan Francisco; Reyes Requena,Antonia; García López,Ana Isabel; Sampaio Cabral,Joaquim Manuel; Fernandes,Pedro; Pina da Fonseca,Luis Joaquim. |
The influence of different commercial surfactants on the enzymatic activity of a commercial alpha-amylase from Bacillus licheniformis (Termamyl 300 L) has been studied. As non-ionic surfactants, alkyl polyglycosides (Glucopon® 215, Glucopon® 600 and Glucopon® 650) were studied, as were fatty alcohol ethoxylates (Findet 1214N/23 and Findet 10/15), and nonyl phenol ethoxylate (Findet 9Q/21.5NF). Also, an anionic surfactant, linear alkyl benzene sulfonate (LAS) was assayed. In general, none of the non-ionic surfactants studied, except Findet 10/15, vary substantially the enzymatic activity. Findet 10/15 has the strongest hydrophobic character and reduces the enzymatic activity more significantly the greater its concentration. Regarding LAS, this surfactant... |
Tipo: Journal article |
Palavras-chave: Alpha-amylase; Alkyl polyglycosides; Enzymatic activity; Fatty alcohol ethoxylates; Linear alkyl benzene sulfonate; Nonyl phenol ethoxylate. |
Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500012 |
| |
|
|
Jia, Honglin; Nishikawa, Yoshifumi; Luo, Yuzi; Yamagishi, Junya; Sugimoto, Chihiro; Xuan, Xuenan. |
The M17 family leucine aminopeptidase (LAP) hydrolyze amino acids from the N-terminus of peptides. Many LAPs from parasitic protozoa including Plasmmodium, Trypanosoma and Leishmania, have been intensely investigated because of their crucial roles in parasite biology. In this study, a functional recombinant Toxoplasma gondii LAP (rTgLAP) was expressed in Escherichia coli and its enzymatic activity against synthetic substrates for aminopeptidase, as well as the cellular localization was determined. Our results indicated that TgLAP is a functional aminopeptidase in the cytoplasma of T. gondii. |
|
Palavras-chave: Toxoplasma gondii; Leucine aminopeptidase; Enzymatic activity. |
Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2822 |
| |
|
|
Wang,Nan; Shi,Haifeng; Yao,Qin; Zhou,Yang; Kang,Lequn; Chen,Huiqin; Chen,Keping. |
Alcohol dehydrogenases (ADH) are a class of enzymes that catalyze the reversible oxidation of alcohols to corresponding aldehydes or ketones, by using either nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP), as coenzymes. In this study, a short-chain ADH gene was identified in Bombyx mori by 5'-RACE PCR. This is the first time the coding region of BmADH has been cloned, expressed, purified and then characterized. The cDNA fragment encoding the BmADH protein was amplified from a pool of silkworm cDNAs by PCR, and then cloned into E. coli expression vector pET-30a(+). The recombinant His-tagged BmADH protein was expressed in E. coli BL21 (DE3), and then purified by metal chelating affinity chromatography. The... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5'-RACE PCRADH; Enzymatic activity; Recombinant protein. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200013 |
| |
|
| |
|
|
Naves,L de P; Corrêa,AD; Bertechini,AG; Gomide,EM; Santos,CD dos. |
The activity of three commercial microbial phytase (Aspergillus oryzae, A. niger, and Saccharomyces cerevisae) products used in broiler nutrition was determined at different pH (2.0 to 9.0) and temperature (20 to 90°C) values. Enzymatic activity was determined according to the reaction of the phytase with its substrate (sodium phytate), in four replicates, and was expressed in units of phytase activity (FTU). A. oryzae phytase exhibited optimal activity at pH 4.0 and 40°C, but its absolute activity was the lowest of the three phytases evaluated. A. niger phytase exhibited maximal activity close to pH 5.0 and 45ºC, whereas S. cerevisae phytase presented its highest activity at pH close to 4.5 and temperatures ranging between 50 and 60°C. It was concluded... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzymatic activity; Enzyme; Phytate; Phytic acid. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2012000300004 |
| |
|
|
Leite,Nadjma Souza; Lima,Aloísio André Bomfim de; Santana,José Carlos Curvelo; Lopes,Francisco Luiz Gumes; Lédo,Ana da Silva; Tambourgi,Elias Basile; Souza,Roberto Rodrigues de. |
This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7). Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL) while in the Imperial... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzymatic activity; Bromelain; Pérola pineapple; Imperial pineapple; Production in vitro. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500002 |
| |
|
|
Duarte,Silvana; Paiva,Marco A. R.; Lara,Camila C.; Bemquerer,Marcelo P.; Araújo,Francisco Gerson. |
The enzymatic activities involved in the digestion of proteins and carbohydrates were compared among three organs of the digestive track of two Siluriformes fish species, and between two areas: a reservoir, and an area downriver of it. Our aim was to test the hypothesis that the digestive organs of species with varied feeding habits have different enzymatic activities, and that the enzymatic activity differs among seasons and environmental conditions. The iliophagous/herbivorous species Hypostomus auroguttatus Kner, 1854 had higher trypsin-like, chymotrypsin-like peptidase and β-glucosidase activity in the intestine when compared with the omnivorous species Pimelodus maculatus Lacepède, 1803, whereas the latter had more hepatic trypsin-like activity than... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzymatic activity; Fishes; Physiology; Reservoirs; Siluriformes; Peptidases; Β-glucosidase. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702013000300006 |
| |
|
| |
Registros recuperados: 29 | |
|
|
|